Bariatric surgery can acutely modulate ER-stress and inflammation on subcutaneous adipose tissue in non-diabetic patients with obesity

Abstract Background Bariatric surgery, especially Roux-en-Y gastric bypass (RYGB), is the most effective and durable treatment option for severe obesity. The mechanisms involving adipose tissue may be important to explain effects of surgery. Methods We aimed identify genetic signatures in patients undergoing RYGB. evaluated 13 obese, non-diabetic (mean age 37 years, 100% women, Body mass index (BMI) 42.2 kg/m 2 ) one day before 3 6 months (M) after Results Analysis gene expression collected at surgery compared with samples M Post-RYGB showed that interleukins [Interleukin 6, Tumor necrosis factor -α ( TNF -α), Monocyte chemoattractant protein-1(MCP1)] endoplasmic reticulum stress (ERS) genes [Eukaryotic translation initiation alpha kinase (EIF2AK3) Calreticulin (CALR)] decreased during follow-up (P ≤ 0.01 all). Otherwise, involved energy homeostasis [Adiponectin AMP-activated protein (AMPK)], cellular response oxidative [Sirtuin 1, Sirtuin 3, Nuclear erythroid 2-related (NRF2)], mitochondrial biogenesis [Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α)] amino acids metabolism [General control nonderepressible (GCN2)] increased from baseline all other time points Also, Peroxisome (PPAR ϒ (adipogenesis regulation) was significantly RYGB < 0.05). Additionally, we observed PGC1α, SIRT1 AMPK strongly correlated BMI all), as well ADIPOQ Conclusions Our findings demonstrate weight loss associated amelioration inflammation ERS protection against tissue. These observations are a decrease essential homeostasis, suggesting an adaptive process on level caloric restriction period Trial registration CAAE: 73,585,317.0.0000.5440